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KMID : 1145720140020020067
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Korean Herbal Medicine Informatics
2014 Volume.2 No. 2 p.67 ~ p.75
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Molecular Authentication of Magnoliae Cortex and Its Adulterant Machilus Cortex based on psbA-trnH DNA Barcode
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Moon Byeong-Cheol
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Abstract
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The original plant species of Magnoliae Cortex (Hu-Bak in Korean and Hou-Po in Chinese) is prescribed as the stem bark of Magnolia officinalis Rehder & E.H.Wilson, Magnolia officinalis var. biloba Rehder & E.H.Wilson, and Magnolia ovobata Thunb. However, the stem bark of Machilus thunbergii Siebold & Zucc. also has been used as a domestic Magnoliae Cortex, To-Hu-Bak. To prevent the distribution of inauthentic Machilus Cortex, we introduced psbA-trnH DNA barcode analysis and established a reliable method for distinguishing between Manoliae Cortex and Machilus Cortex. In comparative analysis of the psbA-trnH sequences using 18 samples of four species, thus, we obtained distinct nucleotide sequences, such as distinct indels (insertions and deletions of nucleotide) and substitutions, enough to differentiate these two herbal materials and evaluated the phylogenetic relationship of these species. The sequence differences at the corresponding alignment positions are useful marker nucleotides to authenticate official Magnoliae Cortex from closely related adulterant of Machilus thunbergii. These marker nucleotides would be useful to standardize the Magnoliae Cortex by the providing of specific genetic information that can distinguish official herbal medicine from inauthentic adulterants for Hu-Bak.
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KEYWORD
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DNA barcode, psbA-trnH, Magnoliae Cortex, Machilus Cortex, Marker Nucleotide
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